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Bpsbioscience/B7-1 (CD80), Fc fusion, Biotin-labeled (Human) HiP™/71114/50 µg

  • 产品编号: 71114
  • 美  元  价: $99999999.99
  • 会  员  价: 待定
  • 品       牌: Bpsbioscience
  • 产       地: 美国
  • 公       司: BPS Bioscience Inc.
  • 产品分类: ref="." class="index">首页 > Bpsbioscience/B7-1 (CD80), Fc fusion, Biotin-labeled (Human) HiP™/71114/50 µg
  • 公司分类: Biotinylation
Size:50 µg
Bpsbioscience/B7-1 (CD80), Fc fusion, Biotin-labeled (Human) HiP™/71114/50 µg
  • Bpsbioscience/B7-1 (CD80), Fc fusion, Biotin-labeled (Human) HiP™/71114/50 µg
  • Bpsbioscience/B7-1 (CD80), Fc fusion, Biotin-labeled (Human) HiP™/71114/50 µg
商品介绍
Species: Human
Host Species: HEK293
MW: 52.5 kDa
Genbank #: NM_005191
Tag(s): C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™
a.a: 35-242
Description:

Human secreted B7-1-Fc fusion protein, also known as CD80, with C-terminal Avi-tag™. GenBank Accession No. NM_005191, a.a 35-242 expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 52.5 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

UniProt
P33681
Synonym(s):
CTLA-4 Counter-Receptor B7.1
Assay Conditions:
Reaction done according to CTLA4:B7-1[biotin] assay kit (Catalog # 72009) protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein was added ON at 4 deg 100 ng/well. Various concentrations of biotinylated ligand (0-8ng/ul, 0.5 ng/ul is the recommended kit condition) added for 2h at RT. Strep-HRP added for 1h at RT.
Formulation:
40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / StABIlity:
At least 6 months at –80°C.
Application(s):
Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Reference(s):

1. Byrum, J.N., Van Komen, J.S., Rodgers, W., J. Immunol. 2013 Sep 15;191(6):3073-3081.

2. Legat, A., et al., Front Immunol. 2013 Dec 19;4:455.

Notes:

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warning(s):
Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.
Scientific Category:
Immunotherapy
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍

BPS Bioscience provides high quality life science products and services to help accelerate drug discovery for the treatment of human diseases.

BPS offers one of the largest selections of unique recombinant proteins and assay kits for the studies of epigenetics, immune checkpoints, metabolism, and cell signaling pathways involved in human diseases. BPS continues to expand its innovative portfolio of products and services, with over 2000 products to advance life science research.

BPS has sold products and services to pharmaceutical companies and academic institutes in over 45 countries worldwide, making us a key resource for immunotherapy research and drug discovery advancement. We maintain a customer-oriented approach and have the capability to customize services to meet specific research needs. Whether the request is for protein expression, screening and profiling services, or assay development, we aim to support our customers in reaching their research goals.


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